Estimation of alpha-amino nitrogen in plasma and urine by the colorimetric ninhydrin reaction
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منابع مشابه
Estimation of Alpha-amino Nitrogen in Plasma and Urine by the Colorimetric Ninhydrin Reaction.
Methods are described for the estimation of plasma and urine alpha-amino nitrogen by the colorimetric ninhydrin reaction; in the method for urine, both ammonia and urea are separated by a rapid ion-exchange resin procedure. Two methods of estimation are given; one has the advantage of great simplicity, but involves the use of cyanide; in the other, cyanide is eliminated. Normal values are repor...
متن کاملThe colorimetric estimation of alpha-amino nitrogen in tissue fluids.
Various authors have reported methods for the colorimetric estimation of alpha-amino nitrogen with ninhydrin (Moore and Stein, 1948, 1954; Smith and Agiza, 1951 ; Troll and Cannan, 1953; Yemm and Cocking, 1955; Kalant, 1956; Rosen, 1957). Only Kalant has applied this method to the direct measurement of alpha-amino nitrogen in biological fluids; he reported on the determination of total alpha-am...
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Of the titrimetric, calorimetric, and gasometric methods now available for determination of amino acids, none is entirely specific. Consequently it has been impossible to tell what proportion of the “amino acid nitrogen” determined in blood or urine with any one of these procedures really represents the amino acids, and what proportion is due to other substances capable of reacting with the rea...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1964
ISSN: 0021-9746
DOI: 10.1136/jcp.17.2.150